Tested Applications
| Positive WB detected in | A375 cells, Raji cells, HepG2 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60154-1-Ig targets SMN (Human-Specific) in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14333 Product name: Recombinant human SMN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-282 aa of BC000908 Sequence: MAMSSGGSGGGVPEQEDSVLFRRGTGQSDDSDIWDDTALIKAYDKAVASFKHALKNGDICETSGKPKTTPKRKPAKKNKSQKKNTAASLQQWKVGDKCSAIWSEDGCIYPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMEMLA Predict reactive species |
| Full Name | survival of motor neuron 2, centromeric |
| Calculated Molecular Weight | 282 aa, 30 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC000908 |
| Gene Symbol | SMN |
| Gene ID (NCBI) | 6607 |
| RRID | AB_10639513 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
| IF protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
| IHC protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
| IP protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
| WB protocol for SMN (Human-Specific) antibody 60154-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMN (Human-Specific) Monoclonal antibody (2C6D9), catalog number 60154-1-Ig, has 4 total citations. These appear in Science, Nature Chemical Biology, Assay and Drug Development Technologies, and RNA. The research fields span spinal muscular atrophy (SMA) gene therapy via base editing, SMN2 splice modulation, SMA drug candidate identification, and U7 snRNA–Sm ring assembly mechanisms.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice. | ||
Assay Drug Dev Technol Assays for the identification and prioritization of drug candidates for spinal muscular atrophy. | ||
RNA Proteins that recognize unique features of U7 snRNA and may substitute for Gemin5 in the assembly of U7-specific Sm ring. |
