"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells, HEK-293 cells, Ag19172 |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66006-2-Ig targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, recombinant protein |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | HA Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | HATAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IF protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| IP protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| WB protocol for HA Tag antibody 66006-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HA Tag Monoclonal antibody (1F5C6), catalog number 66006-2-Ig, has accumulated 524 citations across high-impact journals including Nature, Science, Cell, Cell Metabolism, Immunity, Nature Communications, Molecular Cell, Autophagy, Oncogene, and Advanced Science. Research fields span oncology, autophagy, metabolism, neurology, immunology, virology, cardiovascular disease, liver disease, plant biology, and aging.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
J Hematol Oncol METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma | ||
Science DNA-protein cross-links promote cGAS-STING-driven premature aging and embryonic lethality. |
Reviews
What customers say
Reviews are mixed. Two users rated it 5 stars: one found it works very well for detecting HA tag by Western Blot at 1:5000 dilution, and another praised its performance in immunofluorescence on 293T cells at 1:250, noting low background. However, one user gave a 1-star rating, reporting non-specific labeling when used for Western Blot on HEK cells at 1:10000. Overall, results appear application- and condition-dependent, with strong performance in IF and WB under optimized conditions but potential specificity issues in some settings.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (07-21-2026) | Non-specific labelling
|
Sai Sindhura (Verified Customer) (01-08-2026) | HA tag works very well
|
Parijat (Verified Customer) (03-06-2024) | Good antibody for immunofluorescence. Low background.
|
