Tested Applications
| Positive WB detected in | U-251 cells, human placenta tissue, MCF-7 cells, pig heart tissue, rat heart tissue, U-87 MG cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
| Positive FC (Intra) detected in | HeLa cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66366-1-Ig targets MMP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25039 Product name: Recombinant human MMP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 34-469 aa of BC002576 Sequence: IIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEIC Predict reactive species |
| Full Name | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 55-74 kDa |
| GenBank Accession Number | BC002576 |
| Gene Symbol | MMP2 |
| Gene ID (NCBI) | 4313 |
| RRID | AB_2881746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08253 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MMP2 antibody 66366-1-Ig | Download protocol |
| IF protocol for MMP2 antibody 66366-1-Ig | Download protocol |
| IHC protocol for MMP2 antibody 66366-1-Ig | Download protocol |
| WB protocol for MMP2 antibody 66366-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMP2 Monoclonal antibody (Cat# 66366-1-Ig) has 119 citations across journals including Advanced Materials, Biomaterials, Cell Reports, Cell Death & Disease, Cancer Letters, International Journal of Molecular Sciences, Biochemical Pharmacology, Scientific Reports, and Frontiers in Oncology. Research fields span oncology (hepatocellular, colorectal, ovarian, breast, lung, and gastric cancers), cardiovascular disease (aortic aneurysm, atherosclerosis), fibrosis (pulmonary, hepatic, renal), epithelial-mesenchymal transition, tissue repair, neuroinflammation, and traditional Chinese medicine pharmacology.
| Species | Application | Title |
|---|---|---|
Adv Mater Rejuvenation Modulation of Nucleus Pulposus Progenitor Cells Reverses Senescence-Associated Intervertebral Disc Degeneration | ||
Genes Dis CUDC-907 inhibits glioblastoma and enhances glioblastoma sensitivity to temozolomide by inhibiting DNA damage repair. | ||
Adv Sci (Weinh) Rupestonic Acid of Artemisia Rupestris L. Extract Treats Pulmonary Fibrosis in COPD by Targeting TGF-β1. | ||
Cell Rep Med Mechanical confinement promotes heat resistance of hepatocellular carcinoma via SP1/IL4I1/AHR axis | ||
Biomaterials Cargo-defined engineered vesicles enable targeted miRNA delivery for cardiac repair after myocardial infarction. | ||
Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. |
Reviews
What customers say
All three reviews rate the antibody 4–5 stars for immunohistochemistry. Users report successful staining in skin, zebrafish brain (3 dpf), and retina at dilutions of 1:50 to 1:100. One reviewer noted that PFA fixation without antigen retrieval worked well for both IHC and immunofluorescence in zebrafish tissue. Overall, reviewers describe the antibody as performing reliably for IHC applications across multiple tissue types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cassand (Verified Customer) (04-06-2022) | Works good for IHC
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Ryan (Verified Customer) (02-28-2019) | Tissue was fixed in PFA and no antigen retrieval was used.
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Ryan (Verified Customer) (01-10-2018) |
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