Tested Applications
| Positive WB detected in | MCF-7 cells, Jurkat cells, RAW 264.7 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66400-1-Ig targets GATA3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0174 Product name: Recombinant human GATA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 224-442 aa of BC003070 Sequence: PYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAM Predict reactive species |
| Full Name | GATA binding protein 3 |
| Calculated Molecular Weight | 443 aa, 48 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC003070 |
| Gene Symbol | GATA3 |
| Gene ID (NCBI) | 2625 |
| RRID | AB_2881774 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23771 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GATA3 antibody 66400-1-Ig | Download protocol |
| IHC protocol for GATA3 antibody 66400-1-Ig | Download protocol |
| WB protocol for GATA3 antibody 66400-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GATA3 Monoclonal antibody (Cat# 66400-1-Ig) has 34 citations across journals including Drug Resist Updat, Adv Sci, Cell Death Dis, PNAS, Front Immunol, Int Immunopharmacol, BMC Cancer, and J Ethnopharmacol. Research fields span oncology (breast, bladder, colorectal, and urothelial cancers), immunology (Th2/Treg differentiation, ILC2, T cell function), inflammatory diseases (IBD, asthma, allergic rhinitis), cardiovascular remodeling, liver fibrosis, neurology, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells | ||
Cardiovasc Diabetol BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling | ||
Adv Sci (Weinh) In Situ Transformable Nanoparticle Effectively Suppresses Bladder Cancer by Damaging Mitochondria and Blocking Mitochondrial Autophagy Flux | ||
Adv Sci (Weinh) Targeting ANGPTL3 and IL-33/ST2 Ameliorates Diabetic Kidney Disease by Reducing Lipotoxicity, Alleviating Inflammation and Inhibiting Fibrosis. | ||
Cell Death Dis RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. | ||
Cancer Lett HDAC10 suppresses anti-tumour immunity by inhibiting ILF3-CXCL9 axis to block CD8(+) T cell infiltration and activation in bladder cancer. |
Reviews
What customers say
The single review (rated 4/5) describes using this GATA-3 antibody at 1:100 dilution for immunohistochemistry and immunofluorescence on a 4-μm FFPE lung lymphoid follicle section. After heat-induced antigen retrieval with TRIS-EDTA (pH 9.0) and a tyramide signal amplification (TSA) protocol with an Alexa Fluor 488–conjugated tyramide, the fluorescence staining was clearly noticeable, though the intensity was not very high. Overall, the reviewer found the antibody functional for detecting GATA-3 in tissue, with CD3 co-stained in yellow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Renan (Verified Customer) (08-18-2022) | The fluorescence staining was noticeable, but the intensity was not so high, after using a tyramide signal amplification (TSA) protocol. The picture shows a lung lymphoid follicle, part of a 4-um FFPE tissue section. Heat-induced antigen retrieval was done with TRIS-EDTA buffer (pH 9.0) and GATA-3 was stained using a tyramide-conjugated Alexa Fluor 488 dye. CD3 is stained in yellow.
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