Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells, K-562 cells |
| Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67428-1-Ig targets eIF4G2/DAP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12080 Product name: Recombinant human EIF4G2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 563-907 aa of BC043149 Sequence: EAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4 gamma, 2 |
| Calculated Molecular Weight | 907 aa, 102 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC043149 |
| Gene Symbol | EIF4G2 |
| Gene ID (NCBI) | 1982 |
| RRID | AB_2882667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78344 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for eIF4G2/DAP5 antibody 67428-1-Ig | Download protocol |
| IHC protocol for eIF4G2/DAP5 antibody 67428-1-Ig | Download protocol |
| WB protocol for eIF4G2/DAP5 antibody 67428-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERα antibody (Cat# 67428-1-Ig) has 2 citations. They appear in Cell (2021), studying ERα as an RNA-binding protein sustaining tumor cell survival and drug resistance, and Autophagy (2025), investigating CircRNA GRAMD4-induced NBR1 expression promoting autophagy and immune escape in renal cell carcinoma. Research fields include cancer biology, drug resistance, autophagy, and immune evasion. Applications used were WB and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Cell ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.
| ||
Autophagy CircRNA GRAMD4 induces NBR1 expression to promote autophagy and immune escape in renal cell carcinoma |
