Tested Applications
| Positive WB detected in | A549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0321 Product name: Recombinant human GIT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-462 aa of BC001379 Sequence: DSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQ Predict reactive species |
| Full Name | G protein-coupled receptor kinase interacting ArfGAP 2 |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 70-85 kDa |
| GenBank Accession Number | BC001379 |
| Gene Symbol | GIT2 |
| Gene ID (NCBI) | 9815 |
| RRID | AB_2918616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14161 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GIT2 antibody 67858-1-Ig | Download protocol |
| WB protocol for GIT2 antibody 67858-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GIT2 Monoclonal antibody (2D9C5), catalog number 67858-1-Ig, has a total of 1 citation. It appears in Cancer Letters (Impact Factor 11.8), published in 2024. The cited study investigates how CD24 promotes metastasis and chemoresistance by directly targeting the Arf6-ERK pathway in esophageal squamous cell carcinoma, placing this citation within the fields of cancer biology, tumor metastasis, and chemoresistance.
| Species | Application | Title |
|---|---|---|
Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma. |
