Tested Applications
| Positive WB detected in | A431 cells, K-562 cells, HCT 116 cells, HEK-293 cells, Hela cells, HepG2 cells, Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68040-1-Ig targets SMS in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8840 Product name: Recombinant human SMS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-366 aa of BC009898 Sequence: MAAARHSTLDFMLGAKADGETILKGLQSIFQEQGMAESVHTWQDHGYLATYTNKNGSFANLRIYPHGLVLLDLQSYDGDAQGKEEIDSILNKVEERMKELSQDSTGRVKRLPPIVRGGAIDRYWPTADGRLVEYDIDEVVYDEDSPYQNIKILHSKQFGNILILSGDVNLAESDLAYTRAIMGSGKEDYTGKDVLILGGGDGGILCEIVKLKPKMVTMVEIDQMVIDGCKKYMRKTCGDVLDNLKGDCYQVLIEDCIPVLKRYAKEGREFDYVINDLTAVPISTSPEEDSTWEFLRLILDLSMKVLKQDGKYFTQGNCVNLTEALSLYEEQLGRLYCPVEFSKEIVCVPSYLELWVFYTVWKKAKP Predict reactive species |
| Full Name | spermine synthase |
| Calculated Molecular Weight | 366 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC009898 |
| Gene Symbol | SMS |
| Gene ID (NCBI) | 6611 |
| RRID | AB_2918782 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52788 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SMS antibody 68040-1-Ig | Download protocol |
| IF protocol for SMS antibody 68040-1-Ig | Download protocol |
| WB protocol for SMS antibody 68040-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMS Monoclonal antibody (1G8E9), catalog number 68040-1-Ig, has 1 citation. It appears in Frontiers in Medicine (Lausanne) (2025), in a study investigating how malignant epithelial cells-derived spermine induces APOE+ macrophages to suppress tumor immunity in adenocarcinoma of the esophagogastric junction. The associated research field is tumor immunology and gastrointestinal cancer.
| Species | Application | Title |
|---|---|---|
Front Med (Lausanne) Malignant epithelia cells-derived spermine induces APOE+ macrophages to suppress tumor immunity in adenocarcinoma of the esophagogastric junction |
Reviews
What customers say
The single review for this product reports a 4-star rating from a Western Blot application on mouse liver tissue. The reviewer confirmed the antibody works well at a 1:500 dilution, producing reliable results on mouse liver samples. Overall, the feedback is positive, indicating the antibody performs as expected for WB in mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (12-10-2024) | Works with mouse liver on WB
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