Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83743-2-RR targets Cathepsin D in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1053 Product name: Recombinant Human Cathepsin D protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-412 aa of NM_001909.5 Sequence: LVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL Predict reactive species |
| Full Name | cathepsin D |
| Calculated Molecular Weight | 45kDa |
| Observed Molecular Weight | 32 kDa, 48 kDa, 52 kDa |
| GenBank Accession Number | NM_001909.5 |
| Gene Symbol | Cathepsin D |
| Gene ID (NCBI) | 1509 |
| RRID | AB_3671341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07339 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Cathepsin D antibody 83743-2-RR | Download protocol |
| IF protocol for Cathepsin D antibody 83743-2-RR | Download protocol |
| WB protocol for Cathepsin D antibody 83743-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cathepsin D Recombinant monoclonal antibody (240775E7), catalog number 83743-2-RR, has 1 citation to date. It appears in the journal Autophagy (Impact Factor 18.6), used in a Western blot study on human samples. The research field encompasses autophagy, lipid metabolism, and the AMPK-MTOR signaling pathway in the context of early age-related macular degeneration.
| Species | Application | Title |
|---|---|---|
Autophagy FASN mediates crosstalk between autophagy and lipid metabolism via the AMPK-MTOR pathway in early age-related macular degeneration. |
Reviews
What customers say
The single review for 83743-2-RR reports a 4-star rating from a Western Blot application. The user used 5% milk for blocking, incubated the primary antibody overnight at 4°C, and applied a 1/10000 dilution. The test was performed on 48-hour conditioned media from TNBC (triple-negative breast cancer) cell lines, with two exposure times shown in the accompanying image. Overall, the antibody performed well for detecting the target in this Western Blot setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Isaure (Verified Customer) (05-26-2025) | Sat 5% milk Incubation Primary Ab ON at 4°C (2 exposure times in the uploaded image)
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