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  • Western Blot (WB) analysis of various lysates using EVI2B Recombinant antibody (85943-1-RR)

    WB analysis using 85943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85943-1-RR (EVI2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using EVI2B Recombinant antibody (85943-1-RR)

    IF Staining of THP-1 using 85943-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using EVI2B antibody (85943-1-RR, Clone: 250344C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EVI2B Recombinant antibody

EVI2B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85943-1-RR
Clone No.250344C9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CD361, D17S376, EVDB, EVI 2B, EVI-2B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Ramos cells, THP-1 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85943-1-RR targets EVI2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EVI2B fusion protein Eg3709 Predict reactive species
Full Name ecotropic viral integration site 2B
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC005926
Gene Symbol EVI2B
Gene ID (NCBI) 2124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ecotropic viral integration site 2B protein (EVI2B) is a type I transmembrane protein embedded within intron 27b of the neurofibromatosis type 1 (NF1) gene. It is transcribed in the opposite direction to the NF1 gene (PMID: 37584733). EVI2B is a downstream target of CCAAT/enhancer binding protein alpha (C/EBPα), which is crucial for myeloid differentiation and the function of hematopoietic progenitors (PMID: 36830696). This protein is highly expressed in mature granulocytes and is involved in the regulation of immune cell maturation (PMID: 28186500).

Protocols

Product Specific Protocols
WB protocol for EVI2B antibody 85943-1-RRDownload protocol
IF protocol for EVI2B antibody 85943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols