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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR)

    FC experiment of mouse splenocytes using 98082-1-RR

    1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR, Clone:240230B6) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were stained with CoraLite® Plus 647 Anti-Mouse CD19 before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR)

    FC experiment of mouse splenocytes using 98082-1-RR

    1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR, Clone:240230B6)(red) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98082-1-RR

    Affinity and Kinetic Characterization of 98082-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98082-1-RR against Mouse IFN-beta were performed. The affinity constant is 1.91 nM.

Anti-Mouse IFN-beta Rabbit Recombinant Antibody

IFN-beta Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98082-1-RR
Clone No.240230B6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Ifnb1, 240230B6, Ifb, IFN beta, IFNB

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
PerCP
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC (Intra) detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98082-1-RR targets IFN-beta in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFN-beta fusion protein Eg0660 Predict reactive species
Full Name interferon beta 1, fibroblast
Calculated Molecular Weight22KD
GenBank Accession NumberNM_010510.1
Gene Symbol Ifnb1
Gene ID (NCBI) 15977
RRIDAB_3672229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01575
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.

Protocols

Product Specific Protocols
FC protocol for IFN-beta antibody 98082-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols