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  • Flow cytometry (FC) experiment of mouse thymocytes using Anti-Mouse CD147 Rabbit Recombinant Antibody (98229-1-RR)

    FC experiment of mouse thymocytes using 98229-1-RR

    1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CD147 Rabbit Recombinant Antibody (98229-1-RR, Clone: 241852B4) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse thymocytes using Anti-Mouse CD147 Rabbit Recombinant Antibody (98229-1-RR)

    FC experiment of mouse thymocytes using 98229-1-RR

    1x10^6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CD147 Rabbit Recombinant Antibody (98229-1-RR, Clone: 241852B4) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98229-1-RR

    Affinity and Kinetic Characterization of 98229-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98229-1-RR against Mouse CD147 were performed. The affinity constant is below 1 pM.

CD147 Recombinant antibody

CD147 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98229-1-RR
Clone No.241852B4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Membrane glycoprotein gp42, HT7 antigen, Bsg, Basigin, Basic immunoglobulin superfamily

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1000 μg/ml
100 μg, 1000 μg/ml
Custom

$78/ 100 μg

In stock in Rosemont, IL. Order within 70 hours and 28 mins to receive by Tuesday, May 27

Freight/Packing: $40.00

Quantity

- +

Request bulk or custom quote

Tested Applications

Positive FC detected inmouse thymocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98229-1-RR targets CD147 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name basigin
Calculated Molecular Weight42kDa
GenBank Accession NumberNM_009768.2
Gene Symbol CD147
Gene ID (NCBI) 12215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP18572-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211).

Protocols

Product Specific Protocols
FC protocol for CD147 antibody 98229-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols