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CD200 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2b

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 31.3-2000 pg/mL

Cat No : MP50062-1

Print datasheet

Synonyms

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50062-1, CD200 Monoclonal Matched Antibody Pair - PBS only. 68700-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0006. 68700-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

    Standard curve of MP50062-1

    Sandwich ELISA standard curve of MP50062-1, CD200 Monoclonal Matched Antibody Pair - PBS only. 68700-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0006. 68700-2-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50062-1 targets CD200 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 31.3-2000 pg/mL (Sandwich ELISA);

Capture antibody: CD200 Monoclonal antibody, PBS Only (Capture) 68700-1-PBS (1B1C10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD200 Monoclonal antibody, PBS Only (Detector) 68700-2-PBS (1A1B5). 100 μg. Concentration 1 mgl/ml.

Alternative CD200 matched antibody pairs: MP00030-1, MP00030-2, MP00030-3

Matched pair protein standard: Eg0006

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68700-1-PBS
Tested Reactivity Human
Immunogen Recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD200 molecule
Synonyms CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein
GenBank accession numberBC022522
Gene symbol CD200
UniprotP41217
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68700-2-PBS
Tested Reactivity Human
Immunogen Recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=5.90 x 10-8M
KOff=5.13 x 10-3M
KOn=8.70 x 104M
Full Name CD200 molecule
Synonyms CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein
GenBank accession numberBC022522
Gene symbol CD200
UniprotP41217
Gene ID (NCBI) 4345
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.