Tested Applications
| Positive WB detected in | THP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67067-1-Ig targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5071 Product name: Recombinant human ADRBK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 390-689 aa of BC037963 Sequence: PFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL Predict reactive species |
| Full Name | adrenergic, beta, receptor kinase 1 |
| Calculated Molecular Weight | 689 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC037963 |
| Gene Symbol | ADRBK1 |
| Gene ID (NCBI) | 156 |
| RRID | AB_2882377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25098 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IF protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IHC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| WB protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADRBK1 (β-arrestin 2) Monoclonal antibody (2F7A5), catalog number 67067-1-Ig, has 1 total citation. It appears in the journal Phytotherapy Research (2022), in a study on Paeoniflorin-6'-O-benzene sulfonate protecting the intestinal epithelial barrier via GRK2 and β-arrestin 2. The research field is pharmacology/intestinal barrier biology, with the antibody used in WB, IF, and CoIP on human and mouse samples.
| Species | Application | Title |
|---|---|---|
Phytother Res Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB |
Reviews
What customers say
The antibody performed well in immunofluorescence on RPE1 cells at a 1:400 dilution, producing a clear GRK2 signal (Cy3) with distinct nuclear counterstaining (DAPI). The reviewer tested it across several cell types and was satisfied with the results, awarding a 5-star rating. Overall, the feedback highlights reliable specificity and strong signal quality for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlotte (Verified Customer) (08-05-2024) | I tested the antibody in several cells (here shown RPE1 cells). The GRK2 signal (Cy3) versus the nuclei (Dapi).
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