CoraLite®594-conjugated AHR Monoclonal antibody

AHR Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D1F9

Cat no : CL594-67785

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76



Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67785 targets AHR in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHR fusion protein Ag28935
Full Name aryl hydrocarbon receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Protocols

Product Specific Protocols
FC protocol for CL594 AHR antibody CL594-67785Download protocol
Standard Protocols
Click here to view our Standard Protocols