CoraLite® Plus 488-conjugated AKAP3 Monoclonal antibody

AKAP3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11H9

Cat no : CL488-66255

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

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Validation Data Gallery

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  IF Staining of mouse testis using CL488-66255

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 AKAP3 antibody (CL488-66255, Clone: 3C11H9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66255 targets AKAP3 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKAP3 fusion protein Ag4885
• Full Name: A kinase (PRKA) anchor protein 3
• Calculated Molecular Weight: 95 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC047535
• Gene Symbol: AKAP3
• Gene ID (NCBI): 10566
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.