ALDH5A1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ALDH5A1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B9H10

Cat no : 67347-1-Ig

Synonyms

Succinate-semialdehyde dehydrogenase, mitochondrial, SSDH, SSADH, NAD(+)-dependent succinic semialdehyde dehydrogenase, EC:1.2.1.24

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Validation Data Gallery

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  WB analysis of HepG2 using 67347-1-Ig

  WB result of ALDH5A1 antibody (67347-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.

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  WB analysis of L02 using 67347-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67347-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67347-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67347-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67347-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

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  IF Staining of mouse liver using 67347-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 67347-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ALDH5A1 (67347-1-Ig, Clone:1B9H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, pig brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue, mouse liver tissue
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67347-1-Ig targets ALDH5A1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH5A1 fusion protein Ag11155
• Full Name: aldehyde dehydrogenase 5 family, member A1
• Calculated Molecular Weight: 535 aa, 57 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC034321
• Gene Symbol: ALDH5A1
• Gene ID (NCBI): 7915
• RRID: AB_2882605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.