AOC3 Monoclonal antibody

AOC3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2A6G3

Cat no : 66834-1-Ig

Synonyms

AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP 1, VAP1, Vascular adhesion protein 1



Tested Applications

Positive WB detected inhuman ileum tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:1200
Sample-dependent, check data in validation data gallery

Product Information

66834-1-Ig targets AOC3 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AOC3 fusion protein Ag5908
Full Name amine oxidase, copper containing 3 (vascular adhesion protein 1)
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC050549
Gene symbol AOC3
Gene ID (NCBI) 8639
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).

Protocols

Product Specific Protocols
WB protocol for AOC3 antibody 66834-1-IgDownload protocol
IHC protocol for AOC3 antibody 66834-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols