Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 66206-1-Ig

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag21920
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
IHC protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
IF protocol for Apolipoprotein AI antibody 66206-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

A critical role of VMP1 in lipoprotein secretion.

Authors - Hideaki Morishita
mouseWB

Mol Cell Proteomics

Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.

Authors - Zhuona Rong
human,ratWB

Peptides

Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.

Authors - Bingyang Liu
mouseWB

JCI Insight

Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression.

Authors - Luxiao Li
mouseWB

Front Nutr

Fenofibrate enhances lipid deposition via modulating PPARγ, SREBP-1c, and gut microbiota in ob/ob mice fed a high-fat diet

Authors - Ying Zhang