Validation Data Gallery
|Positive WB detected in||human plasma tissue|
|Positive IHC detected in||human liver cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Apolipoprotein AI fusion protein Ag21920|
|Full Name||apolipoprotein A-I|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||26-28 kDa|
|GenBank accession number||BC005380|
|Gene ID (NCBI)||335|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
|Product Specific Protocols|
|WB protocol for Apolipoprotein AI antibody 66206-1-Ig||Download protocol|
|IHC protocol for Apolipoprotein AI antibody 66206-1-Ig||Download protocol|
|IF protocol for Apolipoprotein AI antibody 66206-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
A critical role of VMP1 in lipoprotein secretion.
Mol Cell Proteomics
Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.
Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.
Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression.
Fenofibrate enhances lipid deposition via modulating PPARγ, SREBP-1c, and gut microbiota in ob/ob mice fed a high-fat diet