CoraLite®488-conjugated Apolipoprotein AI Monoclonal antibody

Apolipoprotein AI Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1C9G5

Cat no : CL488-66206

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66206-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66206 targets Apolipoprotein AI in IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag21920
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-28 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated Apolipoprotein AI antibody CL488-66206Download protocol
Standard Protocols
Click here to view our Standard Protocols