ARH Monoclonal antibody

ARH Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D1B11

Cat no : 66932-2-Ig

Synonyms

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

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Validation Data Gallery

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  WB analysis using 66932-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (ARH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66932-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66932-2-Ig (LDLRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 66932-2-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug ARH Monoclonal antibody (66932-2-Ig, Clone:1D1B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, THP-1 cells, HSC-T6 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66932-2-Ig targets ARH in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARH fusion protein Ag28608
• Full Name: low density lipoprotein receptor adaptor protein 1
• Calculated Molecular Weight: 308 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC029770
• Gene Symbol: ARH
• Gene ID (NCBI): 26119
• RRID: AB_2882258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.