ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 67975-1-Ig

Synonyms

ARHGEF16, GEF16, NBR

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Validation Data Gallery

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  WB analysis using 67975-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67975-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67975-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 67975-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HepG2 cells, Hela cells, HEK-293 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67975-1-Ig targets ARHGEF16 in WB, IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARHGEF16 fusion protein Ag28675
• Full Name: Rho guanine exchange factor (GEF) 16
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC002681
• Gene Symbol: ARHGEF16
• Gene ID (NCBI): 27237
• RRID: AB_2918724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.