ARIH2 Polyclonal antibody, PBS Only

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• ARIH2 Polyclonal antibody, PBS Only (15006-1-PBS)

• 

  WB analysis using 15006-1-AP (same clone as 15006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15006-1-AP (ARIH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15006-1-AP (same clone as 15006-1-PBS)

  IP result of anti-ARIH2 (IP:15006-1-AP, 4ug; Detection:15006-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 15006-1-AP (same clone as 15006-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15006-1-AP (ARIH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15006-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ARIH2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 15006-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

ARI 2, ARI2, ARI-2, ARIH 2, E3 ubiquitin-protein ligase ARIH2

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Product Information

15006-1-PBS targets ARIH2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6507

  Product name: Recombinant human ARIH2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 140-493 aa of BC000422

  Sequence: AVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT

• Full Name: ariadne homolog 2 (Drosophila)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC000422
• Gene Symbol: ARIH2
• Gene ID (NCBI): 10425
• RRID: AB_2290024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95376
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.