Home
Products
Primary Antibodies
ATL3 Polyclonal antibody, PBS Only (16921-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 16921-1-AP (same clone as 16921-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)
WB result of ATL3 antibody (16921-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected HeLa cells. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis using 16921-1-AP (same clone as 16921-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of SMMC-7721 using 16921-1-AP (same clone as 16921-1-PBS)
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 16921-1-AP (same clone as 16921-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 16921-1-AP (same clone as 16921-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)
IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)
IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16921-1-AP (same clone as 16921-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16921-1-AP (same clone as 16921-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 16921-1-AP (same clone as 16921-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 16921-1-AP (same clone as 16921-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)
Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ATL3 Polyclonal antibody, PBS Only

Name: ATL3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 16921-1-PBS

ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16921-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Atlastin 3, Atlastin-3, EC:3.6.5.-

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

16921-1-PBS targets ATL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10279 Product name: Recombinant human ATL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC077727 Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN Predict reactive species
Full Name	atlastin GTPase 3
Calculated Molecular Weight	541 aa, 61 kDa
Observed Molecular Weight	61 kDa
GenBank Accession Number	BC077727
Gene Symbol	ATL3
Gene ID (NCBI)	25923
RRID	AB_2290228
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6DD88
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.