"ATP1A1-Specific Antibodies" Comparison
View side-by-side comparison of ATP1A1-Specific antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | 37°C incubated mouse brain tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, Neuro-2a cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue, human colon tissue, human hepatocellular ca, human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, Caco-2 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| Calculated Molecular Weight | 113 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | NM_000701 |
| Gene Symbol | ATP1A1 |
| Gene ID (NCBI) | 476 |
| ENSEMBL Gene ID | ENSG00000163399 |
| RRID | AB_10859261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ATP1A1-Specific antibody 55187-1-AP | Download protocol |
| IF protocol for ATP1A1-Specific antibody 55187-1-AP | Download protocol |
| IHC protocol for ATP1A1-Specific antibody 55187-1-AP | Download protocol |
| WB protocol for ATP1A1-Specific antibody 55187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP1A1-Specific Polyclonal antibody (Cat# 55187-1-AP) has 44 citations. These appear in journals including Cancer Cell, Cell Metabolism, Immunity, Autophagy, Nature Communications, Circulation Research, Redox Biology, Theranostics, Advanced Science, Hypertension, EMBO Journal, Cell Reports, and PLoS ONE. Research fields span oncology (tumor immunity, metastasis, ferroptosis), metabolism (obesity, insulin resistance), immunology (inflammasome, antigen presentation), neuroscience (Alzheimer's, epilepsy, myelination), cardiology, and ophthalmology, with applications mainly in WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Cancer Cell Inhibiting intracellular CD28 in cancer cells enhances antitumor immunity and overcomes anti-PD-1 resistance via targeting PD-L1 | ||
Cell Metab Discovery of a potent allosteric activator of DGKQ that ameliorates obesity-induced insulin resistance via the sn-1,2-DAG-PKCε signaling axis | ||
Immunity Membrane integrity changes upon viral infection activate sphingomyelinase SMPDL3B to restrict cGAS-STING signaling via cGAMP degradation | ||
Autophagy Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury | ||
Nat Commun Macrophage Gsα promotes NLRP3 stability and its intervention attenuates abdominal aortic aneurysm in male mice. | ||
Nat Commun CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. |
Reviews
What customers say
The single review rates this antibody 5 stars. It was used for immunofluorescence on MDA-MB-231 cells at a 1:100 dilution. Cells were fixed with 2% PFA for 20 minutes, then permeabilized with 0.05% saponin and 5% horse serum in PBS. The primary antibody was incubated at room temperature for 1 hour, followed by a 1-hour incubation with an AlexaFluor 488-conjugated anti-rabbit secondary at 1:200. The reviewer reported successful staining with clear results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Paulina (Verified Customer) (03-26-2025) | Fixation: 2% PFA for 20min. Permeabilization/Antibodies dilution solution: 0.05% saponin, 5% horse serum in PBS. ATP1A1 antibody was diluted 1:100 in saponin solution and incubated on cells at room temperature for 1 hour, followed by 1 hour incubation with anti-rabbit conjugated to AlexaFluor 488 (1:200).
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