|Positive WB detected in||MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:150-1:600|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66037-1-Ig is ATP5A1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||ATP5A1 fusion protein Ag8119|
|Full Name||ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC064562|
|Gene ID (NCBI)||498|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.
|Product Specific Protocols|
|WB protocol for ATP5A1 antibody 66037-1-Ig||Download protocol|
|IHC protocol for ATP5A1 antibody 66037-1-Ig||Download protocol|
|IF protocol for ATP5A1 antibody 66037-1-Ig||Download protocol|
|IP protocol for ATP5A1 antibody 66037-1-Ig||Download protocol|
|FC protocol for ATP5A1 antibody 66037-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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