Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, MDA-MB-231 cells, SK-BR-3 cells, LNCaP cells, HEK-293 cells, K-562 cells, HSC-T6 cells, 4T1 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68326-1-Ig targets AZI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6755 Product name: Recombinant human AZI2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-264 aa of BC001139 Sequence: MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKGKSLFTGNH Predict reactive species |
| Full Name | 5-azacytidine induced 2 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC001139 |
| Gene Symbol | AZI2 |
| Gene ID (NCBI) | 64343 |
| RRID | AB_2935398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H6S1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. NAP1 is a kind of adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. (PMID: 21931631)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AZI2 antibody 68326-1-Ig | Download protocol |
| WB protocol for AZI2 antibody 68326-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AZI2 Monoclonal antibody (2F1G5), catalog number 68326-1-Ig, has 1 citation. It was cited in Nature Communications in a study investigating how NAP1 (AZI2) regulates interferon induction by trapping TBK1 in condensates. The associated research field is innate immunity and interferon signaling, with the antibody used for immunofluorescence (IF) applications.
| Species | Application | Title |
|---|---|---|
Nat Commun NAP1 switches from an activator to a limiter of interferon induction by trapping TBK1 in condensates. |
Reviews
What customers say
The single review rates the antibody 5 stars for Western Blot on HeLa cells at a 1:10,000 dilution. The user tested it in WT versus NAP1 knockout cells and observed two bands, one specific and one nonspecific. The reviewer noted the two bands are very close together and difficult to separate, though overall the antibody performed well for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Muhammed Talha (Verified Customer) (08-31-2023) | I tested this antibody in WT vs NAP1 KO cells. It gave me 2 bands. One of them is actual band and other one is nonspecific one I guess. They are very close to each other and hard to separate them unfortunately.
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