Alpha Actinin Monoclonal antibody, PBS Only

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  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66895-1-Ig (same clone as 66895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (Alpha Actinin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66895-1-Ig (same clone as 66895-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 66895-1-Ig (same clone as 66895-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66895-1-Ig (same clone as 66895-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66895-1-PBS in a different storage buffer formulation.

Alpha Actinin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 66895-1-PBS

Clone No.1E2E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ACTN1, Alpha actinin 1, 1E2E1, actin, actinin, alpha 1

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Product Information

66895-1-PBS targets Alpha Actinin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28383

  Product name: Recombinant human a-Actinin protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 543-892 aa of BC015766

  Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: Alpha Actinin
• Gene ID (NCBI): 87
• RRID: AB_2882224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12814
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.