Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

1E2E1

Cat no : 66895-1-Ig

Synonyms

actinin, alpha 1, ACTN1, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1



Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66895-1-Ig targets Alpha Actinin in WB, IHC applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivitymosquito
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag28383
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol ACTN1
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for Alpha Actinin antibody 66895-1-IgDownload protocol
IHC protocol for Alpha Actinin antibody 66895-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mosquitoWB

Cell Signal

Interplay between autophagy and Sindbis virus in cells derived from key arbovirus vectors, Aedes albopictus and Aedes aegypti mosquitoes

Authors - Jialu Qiao