Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1E2E1

Cat no : 66895-1-Ig

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

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Validation Data Gallery

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  WB analysis using 66895-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66895-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66895-1-Ig (a-Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 66895-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66895-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66895-1-Ig (alpha Actinin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 66895-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Alpha Actinin antibody (66895-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of A431 using 66895-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (66895-1-Ig, Clone:1E2E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C2C12 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

66895-1-Ig targets Alpha Actinin in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, rat, mosquito
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Actinin fusion protein Ag28383
• Full Name: actinin, alpha 1
• Calculated molecular weight: 103 kDa
• Observed molecular weight: 100-105 kDa
• GenBank accession number: BC015766
• Gene symbol: ACTN1
• Gene ID (NCBI): 87
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Publications

Species	Application	Title
mosquito	WB	Cell Signal; Interplay between autophagy and Sindbis virus in cells derived from key arbovirus vectors, Aedes albopictus and Aedes aegypti mosquitoes; Authors - Jialu Qiao
human	IF	Front Pharmacol; Chronic Administration of COVID-19 Drugs Fluvoxamine and Lopinavir Shortens Action Potential Duration by Inhibiting the Human Ether-à-go-go-Related Gene and Cav1.2; Authors - Zequn Zheng
rat	IF	Cardiovasc Diabetol; Dysregulated Rbfox2 produces aberrant splicing of CaV1.2 calcium channel in diabetes-induced cardiac hypertrophy; Authors - Pengpeng Li