Alpha Actinin Monoclonal antibody
Alpha Actinin Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1E2E1
Cat no : 66895-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells |
Positive FC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 2 publications below |
Product Information
66895-1-Ig targets Alpha Actinin in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, rat, mosquito |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Alpha Actinin fusion protein Ag28383 |
Full Name | actinin, alpha 1 |
Calculated molecular weight | 103 kDa |
Observed molecular weight | 100-105 kDa |
GenBank accession number | BC015766 |
Gene symbol | ACTN1 |
Gene ID (NCBI) | 87 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
IHC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
IF protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
FC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Signal Interplay between autophagy and Sindbis virus in cells derived from key arbovirus vectors, Aedes albopictus and Aedes aegypti mosquitoes | ||
Front Pharmacol Chronic Administration of COVID-19 Drugs Fluvoxamine and Lopinavir Shortens Action Potential Duration by Inhibiting the Human Ether-à-go-go-Related Gene and Cav1.2 | ||
Cardiovasc Diabetol Dysregulated Rbfox2 produces aberrant splicing of CaV1.2 calcium channel in diabetes-induced cardiac hypertrophy |