Tested Applications
| Positive WB detected in | HeLa cells, RAW 264.7 cells, HEK-293 cells, pig brain tissue, HepG2 cells, Jurkat cells, HSC-T6 cells, rabbit brain tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66895-1-Ig targets Alpha Actinin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, mosquito |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28383 Product name: Recombinant human a-Actinin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 543-892 aa of BC015766 Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL Predict reactive species |
| Full Name | actinin, alpha 1 |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 100-105 kDa |
| GenBank Accession Number | BC015766 |
| Gene Symbol | Alpha Actinin |
| Gene ID (NCBI) | 87 |
| RRID | AB_2882224 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| IF protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| IHC protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| WB protocol for Alpha Actinin antibody 66895-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alpha Actinin Monoclonal antibody (1E2E1), catalog number 66895-1-Ig, has 16 citations spanning journals including Nature Metabolism, Science Advances, Bioactive Materials, Cellular and Molecular Life Sciences, Journal of Medicinal Chemistry, Phytomedicine, Cardiovascular Diabetology, Journal of Molecular and Cellular Cardiology, International Journal of Biological Sciences, Cell Signaling, Nucleic Acid Therapeutics, Frontiers in Pharmacology, Archives of Pharmacotherapy Research, Cellular and Molecular Biology Letters, International Journal of Molecular Sciences, and Disease Models & Mechanisms. Research fields encompass cardiac hypertrophy, mitochondrial biology, calcium channel signaling, Duchenne muscular dystrophy, oncology, and pharmacology.
| Species | Application | Title |
|---|---|---|
Bioact Mater A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium. | ||
Cardiovasc Diabetol Dysregulated Rbfox2 produces aberrant splicing of CaV1.2 calcium channel in diabetes-induced cardiac hypertrophy | ||
Cell Mol Biol Lett APOE2 protects against Aβ pathology by improving neuronal mitochondrial function through ERRα signaling | ||
Int J Biol Sci Macrophage-derived S100A9 promotes diabetic cardiomyopathy by disturbing mitochondrial quality control via STAT3 activation | ||
Phytomedicine Vitamin K2 (MK7) alleviates arsenic-induced cardiac hypertrophy by suppressing the EGR3/BOK mitochondrial apoptosis pathway. |
