CoraLite®594-conjugated Alpha Actinin Monoclonal antibody

Alpha Actinin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E2E1

Cat no : CL594-66895

Synonyms

actinin, alpha 1, ACTN1, Alpha Actinin, Alpha actinin 1, F actin cross linking protein, FLJ40884, FLJ54432, Non muscle alpha actinin 1

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Validation Data Gallery

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  IF Staining of C2C12 using CL594-66895

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite®594 Alpha Actinin antibody (CL594-66895, Clone: 1E2E1 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66895 targets Alpha Actinin in IF applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Alpha Actinin fusion protein Ag28383
• Full Name: actinin, alpha 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC015766
• Gene Symbol: ACTN1
• Gene ID (NCBI): 87
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.