BBS4 Polyclonal antibody, PBS Only

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• BBS4 Polyclonal antibody, PBS Only (12766-1-PBS)

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  WB analysis using 12766-1-AP (same clone as 12766-1-PBS)

  HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12766-1-AP (same clone as 12766-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 12766-1-AP (same clone as 12766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 12766-1-AP (same clone as 12766-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 12766-1-AP (same clone as 12766-1-PBS)

  IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 12766-1-AP (same clone as 12766-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (12766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 12766-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BBS4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12766-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein

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Product Information

12766-1-PBS targets BBS4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3430

  Product name: Recombinant human BBS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC027624

  Sequence: MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNL

• Full Name: Bardet-Biedl syndrome 4
• Calculated Molecular Weight: 519 aa, 58 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC027624
• Gene Symbol: BBS4
• Gene ID (NCBI): 585
• RRID: AB_10596774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RK4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).