BCL3 Polyclonal antibody, PBS Only

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• BCL3 Polyclonal antibody, PBS Only (23959-1-PBS)

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  WB analysis of HeLa using 23959-1-AP (same clone as 23959-1-PBS)

  WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 23959-1-AP (same clone as 23959-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 23959-1-AP (same clone as 23959-1-PBS)

  IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 23959-1-AP (same clone as 23959-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23959-1-AP (same clone as 23959-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23959-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 23959-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3

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Product Information

23959-1-PBS targets BCL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21040

  Product name: Recombinant human BCL3 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 97-446 aa of BC064993

  Sequence: NLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

• Full Name: B-cell CLL/lymphoma 3
• Calculated Molecular Weight: 446 aa, 47 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC064993
• Gene Symbol: BCL3
• Gene ID (NCBI): 602
• RRID: AB_2879375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20749
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248). The predicted molecular weight of the BCL3 protein is 48 kDa. However, due to post-translational modifications (e.g., phosphorylation), its apparent molecular weight often ranges between 55 and 65 kDa (PMID: 9407099, PMID: 12847243).