Biotin-conjugated BCL6 Monoclonal antibody

BCL6 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin Fluorescent Dye

CloneNo.

1E6B1

Cat no : Biotin-66340

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

Biotin-66340 targets BCL6 in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCL6 fusion protein Ag15519
Full Name B-cell CLL/lymphoma 6
Calculated molecular weight 706 aa, 79 kDa
GenBank accession numberBC150184
Gene symbol BCL6
Gene ID (NCBI) 604
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.

Protocols

Product Specific Protocols
IHC protocol for Biotin BCL6 antibody Biotin-66340Download protocol
Standard Protocols
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