BCL6 Monoclonal antibody

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  WB analysis of Ramos using 66340-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66340-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66340-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BCL6 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66340-1-Ig

Clone No.1E6B1

Publications(8)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

BCL-6, 1E6B1, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

66340-1-Ig targets BCL6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCL6 fusion protein Ag15519
• Full Name: B-cell CLL/lymphoma 6
• Calculated Molecular Weight: 706 aa, 79 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC150184
• Gene Symbol: BCL6
• Gene ID (NCBI): 604
• RRID: AB_2881720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41182
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Publications

Species	Application	Title
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
human	IHC	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IHC，IF	Front Immunol; A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.; Authors - Franziska Werner
human	IHC	iScience; Tertiary lymphoid structure and decreased CD8+ T cell infiltration in minimally invasive adenocarcinoma.; Authors - Jin Wang
mouse	IHC	Cell Death Dis; B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36.; Authors - Hao Zhang
human	IHC	Front Oncol; Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.; Authors - Zhou Qi