|Positive WB detected in||Ramos cells, Raji cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:2000|
|Sample-dependent, check data in validation data gallery|
66340-1-Ig targets BCL6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||BCL6 fusion protein Ag15519|
|Full Name||B-cell CLL/lymphoma 6|
|Calculated molecular weight||706 aa, 79 kDa|
|Observed molecular weight||85 kDa|
|GenBank accession number||BC150184|
|Gene ID (NCBI)||604|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.
Cell Death Dis
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