BCR Monoclonal antibody

BCR Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2E3

Cat no : 67260-1-Ig

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67260-1-Ig targets BCR in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCR fusion protein Ag28953
Full Name breakpoint cluster region
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC066122
Gene Symbol BCR
Gene ID (NCBI) 613
RRIDAB_2882531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for BCR antibody 67260-1-IgDownload protocol
FC protocol for BCR antibody 67260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols