Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2G8B7

Cat no : 67726-1-Ig

Synonyms

CCNB2, Cyclin B, cyclin B2, G2/mitotic specific cyclin B2, HsT17299



Tested Applications

Positive WB detected inDU 145 cells, K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67726-1-Ig targets Cyclin B2 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329
Full Name cyclin B2
Calculated molecular weight 398 aa, 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC105086
Gene symbol CCNB2
Gene ID (NCBI) 9133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

Protocols

Product Specific Protocols
WB protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IHC protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
IF protocol for Cyclin B2 antibody 67726-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols