CoraLite®488-conjugated Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2G8B7

Cat no : CL488-67726

Synonyms

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299



Tested Applications

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 67726-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

CL488-67726 targets Cyclin B2 in IF applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin B2 fusion protein Ag16329
Full Name cyclin B2
Calculated molecular weight 398 aa, 45 kDa
GenBank accession numberBC105086
Gene symbol CCNB2
Gene ID (NCBI) 9133
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.

Protocols

Product Specific Protocols
IF protocol for CL488 Cyclin B2 antibody CL488-67726Download protocol
Standard Protocols
Click here to view our Standard Protocols