CoraLite® Plus 647-conjugated Cyclin B2 Monoclonal antibody

Cyclin B2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G8B7

Cat no : CL647-67726

Synonyms

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

---

Validation Data Gallery

• 

  IF Staining of mouse testis using CL647-67726

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 Cyclin B2 antibody (CL647-67726, Clone: 2G8B7 ) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67726 targets Cyclin B2 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin B2 fusion protein Ag16329
• Full Name: cyclin B2
• Calculated Molecular Weight: 398 aa, 45 kDa
• GenBank Accession Number: BC105086
• Gene Symbol: CCNB2
• Gene ID (NCBI): 9133
• RRID: AB_2920301
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.