Cyclin E2 Polyclonal antibody

Cyclin E2 Polyclonal Antibody for FC, IF, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11935-1-AP

Synonyms

CCNE2, CYCE2, cyclin E2, G1/S specific cyclin E2



Tested Applications

Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11935-1-AP targets Cyclin E2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin E2 fusion protein Ag2532
Full Name cyclin E2
Calculated molecular weight 374 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC020729
Gene symbol CCNE2
Gene ID (NCBI) 9134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.

Protocols

Product Specific Protocols
IHC protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IF protocol for Cyclin E2 antibody 11935-1-APDownload protocol
IP protocol for Cyclin E2 antibody 11935-1-APDownload protocol
FC protocol for Cyclin E2 antibody 11935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Exp Eye Res

Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway.

Authors - Mingyue Zheng
humanWB

Med Sci Monit

Motor Neuron and Pancreas Homeobox 1 (MNX1) Is Involved in Promoting Squamous Cervical Cancer Proliferation via Regulating Cyclin E.

Authors - Li Xiao
humanWB

Neuroreport

Enteric glial cells exert neuroprotection from hyperglycemia-induced damage via Akt/GSK3β pathway.

Authors - Pan Luo
humanWB

Evid Based Complement Alternat Med

Combined Treatment of Cinobufotalin and Gefitinib Exhibits Potent Efficacy against Lung Cancer.

Authors - Youxi Han

Int J Oncol

Tetrandrine induces G1/S cell cycle arrest through the ROS/Akt pathway in EOMA cells and inhibits angiogenesis in vivo.

Authors - Wenkai Xiao
humanWB

Cancer Cell Int

SKA3 promotes cell proliferation and migration in cervical cancer by activating the PI3K/Akt signaling pathway.

Authors - Rong Hu