|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11935-1-AP targets Cyclin E2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin E2 fusion protein Ag2532|
|Full Name||cyclin E2|
|Calculated molecular weight||374 aa, 44 kDa|
|Observed molecular weight||44 kDa|
|GenBank accession number||BC020729|
|Gene ID (NCBI)||9134|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.
|Product Specific Protocols|
|IHC protocol for Cyclin E2 antibody 11935-1-AP||Download protocol|
|IF protocol for Cyclin E2 antibody 11935-1-AP||Download protocol|
|IP protocol for Cyclin E2 antibody 11935-1-AP||Download protocol|
|FC protocol for Cyclin E2 antibody 11935-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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