CoraLite® Plus 647-conjugated Cyclin E2 Polyclonal antibody

Cyclin E2 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11935

Synonyms

CCNE2, CYCE2, cyclin E2, G1/S specific cyclin E2

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Validation Data Gallery

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  IF Staining of HeLa using CL647-11935

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 Cyclin E2 antibody (CL647-11935) at dilution of 1:200.

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  FC experiment of HeLa using CL647-11935

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cyclin E2 (CL647-11935) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-11935 targets Cyclin E2 in IF, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin E2 fusion protein Ag2532
• Full Name: cyclin E2
• Calculated Molecular Weight: 374 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC020729
• Gene Symbol: CCNE2
• Gene ID (NCBI): 9134
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin E2 (CCNE2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of cyclindependent kinases (CDKs). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of cell cycle events. CCNE2 forms a complex with and functions as a regulatory subunit of CDK2 and has been shown to specifically interact with CIP/KIP family of CDK inhibitors. CCNE2 plays a role in cell cycle G1/S transition and its expression peaks at the G1-S phase. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.