Tested Applications
| Positive FC detected in | mouse thymocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
C57BL/6 mouse splenic secondary cytotoxic T cells Predict reactive species |
| Full Name | integrin alpha L |
| GenBank Accession Number | BC145802 |
| Gene Symbol | CD11a |
| Gene ID (NCBI) | 16408 |
| RRID | AB_3084578 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | B7ZN92 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 CD11a antibody CL555-65177 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product reports a 4-star rating for flow cytometry use. The reviewer used a 1:100 overnight staining dilution on freshly isolated mouse cells (spleen, lung, bone marrow, and blood cells), fixed and stained overnight, and found the protocol worked well. Overall, the feedback is positive, confirming reliable performance in FC applications with mouse primary cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Johan (Verified Customer) (01-26-2026) | Freshly isolated mouse cells, fixed and stained O/N.
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