FITC Plus Anti-Mouse CD11a (M17/4)

CD11a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

M17/4

Cat no : FITC-65177

Synonyms

Cd11a, integrin alpha L, Itgal, LFA 1, Ly 15, Ly 21

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65177

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Plus Plus Anti-Mouse CD11a (FITC-65177, Clone: M17/4) (red) or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65177 targets CD11a in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/6 mouse splenic secondary cytotoxic T cells
• Full Name: integrin alpha L
• GenBank Accession Number: BC145802
• Gene Symbol: Itgal
• Gene ID (NCBI): 16408
• RRID: AB_2883815
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).