CoraLite®594-conjugated CD11a/Integrin Alpha L Monoclonal antibody

CD11a/Integrin Alpha L Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E3H12

Cat no : CL594-66256

Synonyms

CD11A, CD11a/Integrin Alpha L, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66256

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66256

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD11a/Integrin Alpha L antibody (CL594-66256, Clone: 1E3H12 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66256 targets CD11a/Integrin Alpha L in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD11a/Integrin Alpha L fusion protein Ag7813
• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: ITGAL
• Gene ID (NCBI): 3683
• RRID: AB_2919972
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).