Biotin Anti-Human CD16a Rabbit Recombinant Antibody

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• Biotin Anti-Human CD16a Rabbit Recombinant Antibody (Biotin-98293)

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  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin, or unstained (blue). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 (B-A19) (CL647-65264, Clone: B-A19). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16a Uni-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-98293

Clone No.242426G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98293 targets CD16a in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2488

  Product name: recombinant human CD16a protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007017 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 29 kDa
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• ENSEMBL Gene ID: ENSG00000203747
• RRID: AB_3746794
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. This antibody is specific for CD16a and does not recognize CD16b.