Biotin Anti-Human CD16a Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin, or unstained (blue). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 (B-A19) (CL647-65264, Clone: B-A19). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98293

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD16a Rabbit RecAb (Biotin-98293, Clone: 242426G11) and PE-conjugated streptavidin (red), or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16a Recombinant Antibody for FC

Cat No. Biotin-98293

Clone No.242426G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98293 targets CD16a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD16a fusion protein Eg2488
• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• ENSEMBL Gene ID: ENSG00000203747
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.