CoraLite®594 Anti-Human CD16a Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL594-98293

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human NCAM1/CD56, and 5 ul CoraLite®594 Anti-Human CD16a Rabbit RecAb (CL594-98293, Clone: 242426G11) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98293

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD16a Rabbit RecAb (CL594-98293, Clone: 242426G11) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16a Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98293

Clone No.242426G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98293 targets CD16a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2488

  Product name: recombinant human CD16a protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007017 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• ENSEMBL Gene ID: ENSG00000203747
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.