FITC Plus Anti-Human CD16a Rabbit Recombinant Antibody

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• FITC Plus Anti-Human CD16a Rabbit Recombinant Antibody (FITC-98293)

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  FC experiment of human PBMCs using FITC-98293

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56, and 5 ul FITC Plus Anti-Human CD16a Rabbit RecAb (FITC-98293, Clone: 242426G11) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98293

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD16a Rabbit RecAb (FITC-98293, Clone:242426G11) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD16a Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-98293

Clone No.242426G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98293 targets CD16a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2488

  Product name: recombinant human CD16a protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007017 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Calculated Molecular Weight: 29 kDa
• Gene Symbol: CD16a
• Gene ID (NCBI): 2214
• ENSEMBL Gene ID: ENSG00000203747
• RRID: AB_3749459
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. This antibody is specific for CD16a and does not recognize CD16b.