CoraLite®700 Anti-Mouse CD206 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using CL700-98031

  1x10^6 mouse peritoneal macrophages were surface stained with CoraLite® Plus 488 Anti-Mouse CD107b / LAMP2, and 0.25 ug CoraLite®700 Anti-Mouse CD206 Rabbit RecAb (CL700-98031, Clone: 240344D12) or unstained. Cells were incubated with FC Receptor Block prior to staining and fixing. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL700-98031

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite®700 Anti-Mouse CD206 Rabbit RecAb (CL700-98031, Clone: 240344D12) (red) or unstained (blue). Cells were incubated with FC Receptor Block prior to staining and fixing. Cells were not fixed.

CD206 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL700-98031

Clone No.240344D12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Mrc1, 240344D12, mannose receptor, C type 1, MMR, MR

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL700-98031 targets CD206 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD206 fusion protein Eg0790
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 165 kDa
• GenBank Accession Number: NM_008625.2
• Gene Symbol: CD206
• Gene ID (NCBI): 17533
• Conjugate: CoraLite®700 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 702 nm / 723 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q2HZ94
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.