Atlantic Blue™ Anti-Mouse LAG-3/CD223 (C9B7W)

• 

  FC experiment of mouse splenocytes using AB-65098

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.25 ug Atlantic Blue™ Anti-Mouse LAG-3/CD223 (C9B7W) (AB-65098, Clone: C9B7W). Cells were not fixed.

LAG-3/CD223 Monoclonal Antibody for FC

Cat No. AB-65098

Clone No.C9B7W

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

CD223, LAG-3, C9B7W, LAG 3, Lag3

---

Tested Applications

• Positive FC detected in: anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

AB-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Murine CD223-Ig fusion protein

• Full Name: lymphocyte-activation gene 3
• GenBank Accession Number: BC120591
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• RRID: AB_2935166
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q61790
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).