CoraLite® Plus 488 Anti-Mouse LAG-3/CD223 (C9B7W)

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  FC experiment of BALB/C mouse splenocytes using CL488-65098

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) BALB/C mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD223 (CL488-65098, Clone: C9B7W). Cells were not fixed.

LAG-3/CD223 Monoclonal Antibody for FC

Cat No. CL488-65098

Clone No.C9B7W

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65098 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Murine CD223-Ig fusion protein

• Full Name: lymphocyte-activation gene 3
• GenBank Accession Number: BC120591
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• RRID: AB_2919262
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q61790
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).