PE Anti-Mouse CD223 (C9B7W)

CD223 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1






PE Fluorescent Dye



Cat no : PE-65098


CD223, LAG 3, Lag3, Ly66, lymphocyte activation gene 3

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65098 targets CD223 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG1
Class Monoclonal
Type Antibody
Immunogen Murine CD223-Ig fusion protein
Full Name lymphocyte-activation gene 3
GenBank accession numberBC120591
Gene symbol Lag3
Gene ID (NCBI) 16768
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).


Product Specific Protocols
FC protocol for PE CD223 antibody PE-65098Download protocol
Standard Protocols
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