Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa


Human and More (1)







Cat no : 65096-1-Ig


CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

65096-1-Ig targets CD25 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name interleukin 2 receptor, alpha
Calculated Molecular Weight 272 aa, 31 kDa
GenBank Accession NumberBC156627
Gene Symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).


Product Specific Protocols
FC protocol for CD25 antibody 65096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



DNA Cell Biol

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Authors - Yuan-Hao Wu